Trifluoroacetic Acid
Adduct Ions
June 20, 2022
Fundamentals of Mass Spectrometry provided by Waters (2 of 7)
Adduct ions are formed by the interaction of a precursor ion with one or more atoms or molecules, such as hydrogen ions, sodium ions, potassium ions, ammonium ions, hydride ions, chloride ions, bromide ions, acetate ions to form an ion.
Adding particular cations or anions into solution can enhance the formation of adduct ions, such as formic acid, acetic acid.
Matrix suppression is the result of adduct ion formation with background ions.
Compounds that readily form particular cations or anions can be added to sample solutions in order to enhance the formation of adduct ions.
This is a transcript of the following video.
...Maximize Sensitivity
October 31, 2022
Buffers for Analyzing Ionizable Compounds
January 3, 2022
This note was copied from Dr. Egidijus Machtejevas' blog on Analytix Reporter, issue 11 | 2022, page 34.
...Choosing Columns and Conditions for the Best Peak Shape
January 3, 2022
Systematic Method Development and Troubleshooting
October 15, 2022
Method Development,
Sensitivity,
Assessment of Matrix Effects,
Trifluoroacetic Acid,
TFA,
Buffer,
Ion Pairs
Tips: Frequently, resolving the problem requires a combination of individual experimental solutions and systematic and logical investigation.
Part 1 highlights rational method development and validation and includes troubleshooting tips for sound sample preparation, for analyte stability in biological matrices, and for chromatographic conditions and injection solvents.
Part 2 further discusses development strategies for LC/MS/MS analysis of biopharmaceutical products including proteins and peptides. It will present troubleshooting tips for carryover, recovery and matrix effects, and selectivity; and for automation where possible to improve the overall reliability and efficiency of LC/MS/MS.
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