Sensitivity
Column Parameters Guide
January 3, 2022
To Compare different core-shell columns.
...Column Selection Showcases
January 3, 2022
I collected some column selection showcases from column manufacturers.
...General Principles of Liquid Chromatography
January 3, 2022
LC Sample Introduction
January 3, 2022
Mobile Phase
January 3, 2022
Mobile Phases
January 3, 2022
- pH can change the retention because the analyte charge could be suppressed, but it can also affect MS response.
- We want to adjust pH to promote the charged state of the analyte in the electrospray ionization mode.
- The concentration of the buffer, or acid or base used to adjust/control the pH, should be as low as possible for ES.
- Ammonium acetate and ammonium formate are generally applicable at pH 7 but concentrations should be kept to a minimum. …