Rule of Three

How Much Retention Time Variation Is Normal?

November 8, 2022
Rule of Three

How Much Retention Time Variation Is Normal?
July 31, 2014
John W. Dolan
LCGC North America, LCGC North America-08-01-2014, Volume 32, Issue 8
Pages: 546–551

Thoughts #

Small change to mobile phase B concentration, column temperature differences, a change in mobile phase A pH, flow rate accuracy and precision, and pump malfunction can cause shifts in retention time.
One of the application of the Rule of Thumb, specifically the Rule of Three is that we can setup an expected/acceptable retention time variation range.

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