APCI General Review
March 17, 2022
The LC elute passing through a nebulizing needle, creates a fine spray. The spray passes through a heated ceramic tube, where the droplets are fully vaporized. The resulting gas/vapor mixture is then passed over a corona discharge needle, where the solvent vapor is ionized to create reagent gas ions.
For successful APCI, the analyte must be volatile and thermally stable and the mobile phase must be suitable for gas-phase acid–base reactions.
To vaporize the solvent and analyte, the APCI source is typically operated at vaporizer temperatures of 400 to 500 0C.
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