Method Development

Systematic Method Development and Troubleshooting

October 15, 2022
Matrix Effects in LC-MS/MS
Method Development, Sensitivity, Assessment of Matrix Effects, Trifluoroacetic Acid, TFA, Buffer, Ion Pairs

 Tips: Frequently, resolving the problem requires a combination of individual experimental solutions and systematic and logical investigation.

 Part 1 highlights rational method development and validation and includes troubleshooting tips for sound sample preparation, for analyte stability in biological matrices, and for chromatographic conditions and injection solvents.

 Part 2 further discusses development strategies for LC/MS/MS analysis of biopharmaceutical products including proteins and peptides. It will present troubleshooting tips for carryover, recovery and matrix effects, and selectivity; and for automation where possible to improve the overall reliability and efficiency of LC/MS/MS.

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