Fundamentals of Mass Spectrometry provided by Waters (2 of 7)
Adduct ions are formed by the interaction of a precursor ion with one or more atoms or molecules, such as hydrogen ions, sodium ions, potassium ions, ammonium ions, hydride ions, chloride ions, bromide ions, acetate ions to form an ion.
Adding particular cations or anions into solution can enhance the formation of adduct ions, such as formic acid, acetic acid.
Matrix suppression is the result of adduct ion formation with background ions.
Compounds that readily form particular cations or anions can be added to sample solutions in order to enhance the formation of adduct ions.
Tips: Frequently, resolving the problem requires a combination of individual experimental solutions and systematic and logical investigation.
Part 1 highlights rational method development and validation and includes troubleshooting tips for sound sample preparation, for analyte stability in biological matrices, and for chromatographic conditions and injection solvents.
Part 2 further discusses development strategies for LC/MS/MS analysis of biopharmaceutical products including proteins and peptides. It will present troubleshooting tips for carryover, recovery and matrix effects, and selectivity; and for automation where possible to improve the overall reliability and efficiency of LC/MS/MS.