ESI APCI chart

02 Method Development

July 13, 2022
ESI APCI chart, three MS major parameters, five ion source parameters

Video: 02 Method Development

Step 1: Compound optimization

  1. Q1 scan (identify the parent)
  2. Product ion scan (identify the fragments)
  3. MRM scans (optimize your parameters)

Step 2: Chromatography

  1. Choosing a column
  2. Selecting a mobile phase
  3. Using chromatography

Step 3: sMRM method

  1. Run a mid or high level standard
  2. Create a method in analytics and process a sample
  3. Copy and paste RT into your method
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