October 15, 2022
Tips: Frequently, resolving the problem requires a combination of individual experimental solutions and systematic and logical investigation.
Part 1 highlights rational method development and validation and includes troubleshooting tips for sound sample preparation, for analyte stability in biological matrices, and for chromatographic conditions and injection solvents.
Part 2 further discusses development strategies for LC/MS/MS analysis of biopharmaceutical products including proteins and peptides. It will present troubleshooting tips for carryover, recovery and matrix effects, and selectivity; and for automation where possible to improve the overall reliability and efficiency of LC/MS/MS.
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March 17, 2022
Conclusion:
The Turbo Ion Spray (off-axis geometry), Turbo VTM Source (orthogonal geometry) and the Standard Z-Spray Source (double orthogonal geometry) did not differ much in their susceptibility to MEs.
The Jet Stream ESI (orthogonal geometry) reaches a higher sensitivity by an additional heated sheath gas, but suffers at the same time from significantly stronger signal suppressions than the comparable Standard ESI (orthogonal geometry) without sheath gas. No relation between source geometry and extent of signal suppression was found in this study.
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March 17, 2022
Strategies for the Detection and Elimination of Matrix Effects in Quantitative LC–MS Analysis
January 13, 2014
Swee K. Tan, P. Nicholas Shaw, Amitha K. Hewavitharana
LCGC North America, LCGC North America-01-01-2014, Volume 32, Issue 1
Pages: 54–64
We assessed a simple method based on recovery for the detection of matrix effects and two alternative methods for the rectification of matrix effects in LC–MS: standard addition and the coeluting internal standard method.
Currently available methods for the detection of matrix effects in liquid chromatography–mass spectrometry (LC–MS) are tedious and complex; therefore, a simpler method is required. Although there are no methods to completely eliminate matrix effects, the most well-recognized technique available to correct for matrix effects is that of internal standardization using stable isotope–labeled versions of the analytes. As this method can prove expensive, an alternative method of correction is likely to be useful. In this study, a simple method based on recovery is assessed for the detection of matrix effects. Two alternative methods for the rectification of matrix effects in LC–MS are also assessed: standard addition and the coeluting internal standard method.
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