Column Capacity

Sigma-Aldrich Guide #

Column capacity depends on many factors, but typical values for total amounts of analytes on a column are:

**Table 2. Approximate Column Volumes for some Popular Column Dimensions and Their Multiples**
Length (mm)	ID (mm)	Approximate Column Volume (mL)	10 Column Volumes (mL)	15 Column Volumes (mL)
250	4.6	4.15	41.5	62.3
250	2.0	0.79	7.9	11.8
150	4.6	2.49	24.9	37.4
150	2.0	0.47	4.7	7.1
100	4.6	1.66	16.6	24.9
100	2.0	0.31	3.1	4.7
50	4.6	0.83	8.3	12.5
50	2.0	0.16	1.6	2.4
25	4.6	0.42	4.2	6.2
25	2.0	0.08	0.8	1.2

**Table 3: Column Volumn Equations**
Type of Columns	Equation
Fully Porous	$V = 0.68 \times \pi \times r^2 \times L $
Superficially Porous	$V = 0.50 \times \pi \times r^2 \times L $