phenomenex - Overcoming the Challenges of Sample Preparation and LC/MS/MS Method Development

Learning objectives

How to determine the best sample preparation technique
Method development tips for achieving separation of target compounds via LC/MS/MS
How to increase throughput without sacrificing results

Isobaric interference example:

Figure 1: Known Interference

Unknown interference example:

Figure 2: Unknown Interference

Matrix effect example:

Figure 3: Matrix Effect

What contributes to the matrix effect?
- Disease state
- Endogenous compounds
- Exogenous compounds
- MS source design
- Sample preparation