I collected some column selection showcases from column manufacturers.
Agilent Column Selection Guide #
| General Phase Type | Starting ZORBAX Choice |
|---|---|
| Typical “endcapped” C18 or C8 | |
| bonded phases, newer columns | Eclipse Plus C18 or C8 |
| Endcapped C18 or C8 columns, | |
| older generation | Eclipse XDB-C18 or C8 |
| Non-endcapped columns | StableBond C18 |
| Older types of columns | StableBond C18, C8 etc. |
| Aqueous “type” columns | SB-AQ |
| CN or Phenyl | SB-CN, SB-Phenyl |
Thermo Column Selection Guide #
Thermo Scientific reversed phase materials are based on high purity silicas giving excellent chromatography of acids, bases, and neutral compounds. Our premier columns include Hypersil GOLD and BioBasic families in a wide range of chemistries, an excellent choice for all of your chromatography needs. Choose any of our families for method development, LC/MS, and your most demanding separations. All of our reversed phase materials offer superb reproducibility and reliable performance for routine and QC analyses. Our experienced Technical Support staff are always able to help you select the appropriate column for your application.
Premium Columns #
Hypersil GOLD™ Columns: The key to outstanding peak shape
• Exceptional peak symmetry and resolution
• Based on next-generation ultra pure silica
• Outstanding pH stability
• Ideal first choice for new method development
BioBasic™ Columns: Better chromatography of biomolecules
• 300Å pore size for better protein and peptide separations
• Excellent reproducibility, efficiency, and column lifetimes
• Range of stationary phases to optimize selectivity, including ion
exchange and size exclusion
• Ideal for proteomics and LC/MS of biomolecules
• Excellent alternative to Vydac®, YMC-Pack™ and Zorbax® wide pore
columns
Classical Hypersil™ Columns #
Thermo Scientific Classical Hypersil reversed phase chemistries provide a proven record of performance for routine analyses year after year. Specified in thousands of methods worldwide, our classical Hypersil families offer exceptional reproducibility and reliability.
Hypersil BDS Columns: The standard base deactivated column
• Base deactivated for reduced peak tailing and excellent peak
symmetry
• Highly reproducible and efficient
• Reliable with long column lifetimes
• Excellent performance with basic, acidic and neutral compounds
Hypersil Classical Columns: Reliable and reproducible
• Excellent phase used for thousands of existing methods
• High efficiency, proven reproducibility, and long column lifetimes
• Wide range of bonded phases including dedicated columns for specific
applications
Performance Columns #
HyPURITY™ Columns: The choice for superior chromatography
• Ultra-pure, highly stable silica for long column lifetime, reliability
and performance
• Excellent peak shapes for acids, bases, chelators and neutral
compounds
• 190Å pore size for small molecules, peptides, and protein digests
• Family of phases for a variety of selectivities
• Ideal for LC/MS applications
• Excellent alternative to other highly pure silica columns
BetaBasic™ Columns: Stable, inert and reproducible for general HPLC
• Superb stability at both high and low pH
• Efficient and reproducible columns
• A family of useful stationary phases
• 150Å pore size for small molecules, peptides and protein digests
BETASIL™ Columns: High retention, efficiency and selectivity
• Highly retentive phases with excellent peak shapes and lifetimes
• High surface area with high bonded phase coverage
• Phases with unique selectivity for reversed phase and normal phase
• Ideal for LC/MS applications, including short, fast DASH™ columns
• Excellent substitute for Inertsil® columns
BetaMax™ Columns: Maximum retention for maximum results
• Ultra-high surface area and surface coverage for maximum retention
• 3 chemistries for 3 types of analytes: acid, base, and neutral
• Excellent stability for long column lifetimes
• BetaMax Neutral provides maximum C18 reversed phase retention for
LC/MS applications
Unique Selectivities #
Hypercarb™ Columns: 100% porous graphitic carbon solves problematic
separations
• Exceptional retention of polar compounds
• pH stable from 1 to 14
• Separate structurally similar compounds
• Ideal for LC/MS applications other non-silica based columns
Hypersil GOLD aQ Columns: Retention of polar compounds • Polar end-capped reversed phase columns for enhanced retention of polar compounds • Compatible with 100% aqueous mobile phases and standard reversed phase mobile phases • Run with reduced buffer concentrations • C18 phase for reliability and high performance.
Hypersil GOLD PFP Columns: Fluorinated phases for highly selective reversed phase chromatography • Fluorinated phase provides unique selectivity • Extra retention and selectivity for fluorinated and chlorinated compounds • Excellent selectivity for polar nonhalogenated compounds with nitro, hydroxyl, carboxyl and other polar groups • Shape selective for structural isomers • Use typical reversed phase solvents and conditions
Hypersil GOLD Phenyl Columns: Alternative selectivity for aromatic and moderately polar analytes • Butyl linker provides enhanced pi-pi interactions with aromatics • Moderate hydrophobicity • Outstanding peak shape and sensitivity
HyPURITY ADVANCE™ Columns: Polar embedded phase for reversed phase chromatography • Unique reversed phase selectivity • Ideal for the separation of polar compounds
Shodex Column Selection Guide #
