To Compare different core-shell columns.

Column Parameters (Bonded Phase Type, L, d_p, d_c) #

While the C₁₈ stationary phase is traditionally used, the selectivity of different bonded phases is often exploited for the separation of closely eluting pairs of key analytes in critical analyses. Column screening with a variety of stationary phases that offers different retention mechanisms can occur during either initial method development or the fine-tuning steps but may be dependent on the availability of suitable samples. Automated column/mobile phase screening systems equipped with multiport selection valves (Figure 10.5) are typically used with an “orthogonal” column set (e.g. C₁₈ ( C₈), C₁₈ AQ, phenyl, cyano, polar-embedded, and pentafluorophenyl).

Column dimensions (L, d_p, d_c) are matched to the intended application and can be optimized to enhance efficiency, speed, or sensitivity. One useful strategy is to use short UHPLC columns (50 × 3.0 mm, sub-3-μm) for rapid column/mobile phase screening, followed by optimization with longer columns (to maximize resolution) using the selected bonded phase [5]. SPPs are preferred in RPC because of the higher efficiency as compared to similar totally porous particles (TPPs) [10].

Source: Michael W. Dong, HPLC and UHPLC For Practicing Scientists, 2nd Edition, P255