Category

Hydrophilic-Lipophilic Balance

January 3, 2022
Category

Figure :

The hydrophilic-lipophilic balance is a measure of the degree to which it is hydrophilic or lipophilic (or hydrophobic), determined by calculating values for the different regions of the molecule, as described by Griffin in 1949 and 1954. Other methods have been suggested, notably in 1957 by Davies. source: wiki

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LC column Selection Guide

January 3, 2022
Category

Figure :

Here is some basic principle for HPLC column choice.

  • Matrices / Substrate
  • Stationary phase / Ligand
  • Particle diameter
  • Pore size
  • column size
  • Guard columns
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